Figure 2. p21 content in HMVEC after CXCL4^47–70 and CXCL4L1^47–70 stimulation.

The impact of CXCL4^47–70 and CXCL4L1^47–70 on the cell cycle in HMVEC was evaluated by quantification of p21 production. Intracellular p21 concentrations, known to drop upon EGF stimulation (20 ng/ml), were also evaluated in HMVEC stimulated with EGF combined with either CXCL4^47–70 (0.3 to 10 μg/ml) or CXCL4L1^47–70 (0.3 to 3 μg/ml). The ratio of p21: total protein content was calculated for cell lysates after 15h incubation with various stimuli. Results (mean ± s.e.m.) are expressed relative to the p21 content after control treatment (100%; dotted line). n= 4 to 9; **p<0.01 (EGF versus CO); $p<0.05,$$p<0.01 (versus EGF)