Figure 4. Proliferation of MDA-MB-231 cells examined after CXCL4^47–70 and CXCL4L1^47–70 stimulation.

The anti-proliferative effect of CXCL4^47–70 and CXCL4L1^47–70 on MDA-MB-231 tumor cells in vitro was investigated performing the MTT proliferation assay. Over the course of 3 to 4 days, MDA-MB-231 cells were incubated with either control treatment (CO), CXCL4^47–70 (0.1 to 10 μg/ml) or CXCL4L1^47–70 (0.1 to 30 μg/ml). Afterwards cell cultures were analyzed with the MTT assay protocol. n= 3 to 7, tested in triplicate per experiment; *p<0.05, **p<0.01 (versus CO)