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In order to evaluate interaction of CXCL447–70 and CXCL4L147–70 with EGF, binding of amino-terminally biotinylated CXCL447–70 or CXCL4L147–70 to EGF-coated plates was detected with peroxidase-conjugated streptavidin. Results are expressed as average optical densities (± s.e.m.) measured at 450 nm (OD450). n= 3
