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. 2014 Oct 21;5(21):10916–10933. doi: 10.18632/oncotarget.2538

Table 1. Binding parameters of the interaction of CXCL447–70 and CXCL4L147–70 peptides with heparin immobilized on a BIAcore sensorchip.

Peptide Association rate (Kon) (1/Ms) Dissociation rate (Koff) (1/s) Kd (Koff/Kon) (M) Kd (M) at equilibrium
CXCL447–70 6.7 × 105 1.1 × 102 1.6 × 108 7.2 × 108
CXCL4L147–70 5.2 × 104 5.3 × 102 1.0 × 106 1.2 × 106

Kon and Koff of peptide/heparin interactions are shown. The Kd value was derived from the Koff/Kon ratio as well as by Scatchard plot analysis of the equilibrium binding data.