Table 1. Binding parameters of the interaction of CXCL447–70 and CXCL4L147–70 peptides with heparin immobilized on a BIAcore sensorchip.
Peptide | Association rate (Kon) (1/Ms) | Dissociation rate (Koff) (1/s) | Kd (Koff/Kon) (M) | Kd (M) at equilibrium |
---|---|---|---|---|
CXCL447–70 | 6.7 × 105 | 1.1 × 10−2 | 1.6 × 10−8 | 7.2 × 10−8 |
CXCL4L147–70 | 5.2 × 104 | 5.3 × 10−2 | 1.0 × 10−6 | 1.2 × 10−6 |
Kon and Koff of peptide/heparin interactions are shown. The Kd value was derived from the Koff/Kon ratio as well as by Scatchard plot analysis of the equilibrium binding data.