Table 2. Primer and probe sequences for qPCR assays.

Gene	Primers (5′→ 3′)	Probe (5′→ 3′)
F4/80	GGAAGTGGATGGCATAGATGA ATTCACTGTCTGCTCAACCG	/HEX/AGTCTGGGA/ZEN/ ATGGGAGCTAAGGTCA/3IABkFQ/
IFN-γ	TCCACATCTATGCCACTTGAG CTGAGACAATGAACGCTACACA	/HEX/TCTTGGCTT/ZEN/ TGCAGCTCTTCCTCA/3IABkFQ/
TGM2	GGTTCATATCCAAGAGCATCAGG GTCAAGTTCATCAAGAGTGTGC	/FAM/TTCCATCCT/ZEN/ CGAACTGCCCAAAGT/3IABkFQ/
CD204	CACAAGGAGGTAGAGAGCAATG GCACGTTCAATGACAGCATC	/FAM/TCACAGCAC/ZEN/ TAAAAATGGCCCCTCC/3IABkFQ/
IL-12	TCCAGTCCACCTCTACAACA TGTCCTCAGAAGCTAACCATC	/FAM/ACGTCTTTC/ZEN/ TCCAGCTCCCACATG/3IABkFQ/
NOS2	CACTTCTGCTCCAAATCCAAC GACTGAGCTGTTAGAGACACTT	/HEX/TGAACAAGA/ZEN/ CCCAAGCGTGAGGAG/3IABkFQ/
CD11c	CTACCCGAGCCATCAATCAG GCTCTGCTTTCTACTGAGTTCA	/HEX/AGCCAGAAC/ZEN/ TTCCCAACTGCACA/3IABkFQ/
CCL5 (mu)	CCTCTATCCTAGCTCATCTCCA GCTCCAATCTTGCAGTCGT	/HEX/TCTTCTCTG/ZEN/ GGTTGGCACACACTT/3IABkFQ/
CCL5* (hu)	TGCCACTGGTGTAGAAATACTC GCTGTCATCCTCATTGCTACT	/FAM/ATCTGCCTC/ZEN/ CCCATATTCCTCGGA/3IABkFQ/
18S	ATCGCTCCACCAACTAAGAAC ACGGACAGGATTGACAGATTG	/FAM/ACCACCCAC/ZEN/ GGAATCGAGAAAGAG/3IABkFQ/

All primer/probe sets were supplied as PrimeTime qPCR assays by Integrated DNA Technologies (IDT)

^*All assays were designed for murine (mu) gene products with the exception of this assay, designed to detect the human (hu) CCL5 cDNA.