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. 2014 Apr 24;5(21):10949–10958. doi: 10.18632/oncotarget.1919

Figure 3. Activation marker upregulation and cell proliferation assay.

Figure 3

(A) Transduced PBLs with either N1/28z or NGFR cells were co-cultured with tumor lines as indicated and analyzed by flow cytometry for marker expression gated on the CD8+ population (CD25 – left panels; CD69 – right panels). The percentage of positive cells and the MFI (in brackets) are shown and the dotted line represents the marker staining of the NGFR control. These results are representative of at least four independent experiments with at least three donors and the difference between N1/28z and NGFR was found to be statistically significant (p<0.05, calculated using a Student's paired t-test). (B) Proliferation assay. CFSE-labeled activated transduced T-cells were stimulated with plate-bound anti-NCR1 (0.5 ug/well). Two and 4 days after stimulation, the cells were analyzed for CFSE dilution. The MFI is shown at the different time points. These results are representative of three independent experiments with two donors and the difference between the two groups was found statistically significant (p<0.05, calculated using a Student's paired t-test).