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. 2014 Nov;5(11-12):407–419. doi: 10.18632/genesandcancer.44

Figure 5. DEPTOR knockdown-induces P21 and P27 in a P53-independent fashion.

Figure 5

A) Transfected cells treated +/− dox followed by immunoblot. B) Inducible shDEPTOR cells ectopically expressing scramble or RAPTOR shRNAS treated +/− dox followed by immunoblot; C) Inducible shDEPTOR cells ectopically expressing EV or AKT(WT) or phosphomimetic AKT treated+/− dox followed by immunoblot; D) qt-PCR for p21 RNA expression following infection with control (shLuc) or DEPTOR shRNA and treated+/− dox (mean+/− SD, n=3). E) Acute infection with shRNA scramble (control) or DEPTOR shRNA in OPM-2 cells followed by immunoblot; F) Mice (8/group) challenged with SQ injection of inducible 8226 cells and, when tumor size reached 400 mm3, mice were randomized to receive dox in water or no dox. Tumor size is mean+/−SDs. G) Western blot of tumor protein extracted from 2 separate mice from each group.