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. 2014 Nov;5(11-12):407–419. doi: 10.18632/genesandcancer.44

Figure 8. Use of shRNA-resistant DEPTOR.

Figure 8

A) 8226 MM cells transfected with empty vector (EV), mutant DEPTOR (resistant) or wild type (WT) DEPTOR and either treated with or without shRNA targeting DEPTOR, followed by immunoblot; B) % apoptosis (mean+/− SD, n=3) following DEPTOR knockdown (+/−shRNA) in EV, mutant-DEPTOR (resistant) or wild type DEPTOR-transfected 8226 cells. C) qt-PCR quantification of relative amounts of miRNA 106a, 93 and 20b in MM cells transfected as in ‘A’ (EV=empty vector; WT=wild type DEPTOR; Mu=mutant (resistant) DEPTOR). Data are means+/−SD, n=3. D) Model of events following DEPTOR knockdown in MM cells.