Skip to main content
. Author manuscript; available in PMC: 2014 Dec 30.
Published in final edited form as: Curr Pharm Des. 2012;18(27):4187–4196. doi: 10.2174/138161212802430486

Fig. (3). Tag-free ILYd4 has better physical properties and anti-hCD59 activity than His-tagged ILYd4.

Fig. (3)

(A) Solubility of His-tagged ILYd4 and tag-free ILYd4 in three different pH buffers (pH 5.0, 7.4, and 9.0). We used 15% SDS-PAGE to analyze the fractions - solution (S) and pellet (P) (total 5μg) obtained after centrifugation of the protein dialyzed against three different pH conditions. (B) Native gel (10%) analysis of the aggregation state of the tag-free and His-tagged ILYd4 (5μg) dialyzed against pH 5.0 and 7.4 buffer. (C) Anti-hCD59 activity of the-tag free and His-tagged ILYd4 dialyzed against pH 5.0 buffer by complement-mediated assays on human erythrocytes with 10% (v/v) NHS as a source of complement. (D) ED50 of tag-free ILYd4 for human erythrocytes was three-fold lower than that of His-tagged ILYd4 in pH 5.0 buffer. Results are mean ± SD of three independent experiments. *: P < 0.01 v.s. His-tagged ILYd4.