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. Author manuscript; available in PMC: 2014 Dec 30.
Published in final edited form as: Curr Pharm Des. 2012;18(27):4187–4196. doi: 10.2174/138161212802430486

Fig. (6). No significant change in the solubility of His-tagged and tag-free ILYd4 under the different temperatures.

Fig. (6)

After storing the proteins in PBS buffer in 25°C or 40°C at different time periods, we used 15% SDS-PAGE to determine the relative amount of protein in solution (S) and pellet (P) (total 5μg) obtained following centrifugation of the dialyzed proteins. (A). The solubility of tag-free ILYd4 by assessing their relative distribution after storing in PBS buffer in either 25°C or 40°C over different time periods. (B). The solubility of His-tagged ILYd4 by assessing their relative distribution after storing in PBS buffer in either 25°C or 40°C over different time periods.