Skip to main content
. 2014 Jun 5;26(1):67–80. doi: 10.1681/ASN.2013101045

Figure 4.

Figure 4.

Deletion of RBP-J increases the number of JG cells that express neither renin nor SM markers. (A) Double staining for renin (brown) and α-SMA (purple) shows that cKO mice have few or no renin-positive JG cells (arrows) and diminished or absent α-SMA expression in JGAs. *Glomeruli. (B) Five cells in each JGA were counted and categorized as (1) renin+ only, (2) α-SMA+ only, (3) both renin+ and α-SMA+, and (4) not stained. (C) A summary of the number of cells in each category shows that RBP-J cKO mice had an increased number of unstained cells. (D) The number of JGAs with one to five cells unstained with either antibody. RBP-J cKO mice had more JGAs with two to five unstained cells, whereas the number of JGAs with one unstained cell is similar in RBP-J cKO and control animals. In total, 219 JGA cells were counted in the controls (n=2), and 216 JGA cells were counted in the mutants (n=2). Each tick indicates a JGA. Cells in yellow are cells stained with either antibody.