. 2014 Jun 5;26(1):209–219. doi: 10.1681/ASN.2013121339

Table 1.

MLPA probes used to determine CFH-CFHR copy number

Probe No.	Probe Name	Hybridization Sequence (20 Nucleotides Adjacent to Ligation Site)
1	CFH exon 1	TGCTACACAA-ATAGCCCATA
2	CFH exon 2	GGTCTGACCA-AACATATCCA
3	CFH exon 3	TCCTTTTGGT-ACTTTTACCC
4	CFH exon 4	ATTACCGTGA-ATGTGACACA
5	CFH exon 6	AAAGAGGAGA-TGCTGTATGC
6	CFH intron 10	TAGGTAGTCA-TATTTGGAAC
7	CFH intron 12	TGGACACATT-ATGATTGAGT
8	CFH exon 13	AGTTGGACCT-AATTCCGTTC
9	CFH exon 18	GGAACCATTA-ATTCATCCAG
10	CFH exon 19	AGGATGTGTA-TAAGGCGGGG
11	CFH intron 20	GAATTCTATT-TACACTTCCG
12	CFH intron 21	TAATAGGGTA-TATTATTTTC
13	CFH intron 22	GAAAAATCTC-TGTGATGAGT
14^a	CFH exon 23	AGCTTTATTC-GAGAACAGGT
15^a	CFH intron 23	TCAATACATA-AATGCACCAA
16^a	CFH intron 23	CACTTATACA-TGCAATCCGT
17^a	CFH intron 23	AGTCCGAGGT-AGAAAGGGAC
18^a	CFH intron 23	GTGGTAATCT-TGGCTCTCAG
19^a	CFHR3 intron 1	AGGTAAGTTA-AAAGAGATCT
20^a	CFHR3 intron 1	CATTTTCTTG-TGGAATTACAGC
21^a	CFHR3 intron 3	CGGACGACAG-TCTCAGACTT
22^a	CFHR3 intron 4	GGGTTATATG-AATTCCTACA
23^a	CFHR3 exon 6	TCCCTTCCCG-ACACACTGCTTG
24^a	CFHR1 intron 3	AGAGTTTCAG-GTCCATGTGT
25^a	CFHR1 intron 5	AATCTGTGAT-TATTTTGTTA
26	CFHR1 exon 6	CCTGTTCTCA-AATAAAGCTTCT
27	CFHR1 exon 6	TTTTCCAAGT-TTTAATATGG
28	CFHR2 intron 1	TGTCTGTACT-TGGAGTTTCG
29	CFHR2 intron 2	AGATCATAAA-CACTTGATAA
30	CFHR2 intron 3	AATACCTGTG-TGTGGTTTATAG
31	CFHR2 exon 4	ATATGCTCCAGG-TTCATCAGTT
32	CFHR5 exon 1	TGGGTATCCA-CTGTTGGGGG
33	CFHR5 exon 2	TGAAGAAGAT-TATAACCCTT
34	CFHR5 exon 3	CTTCAGGACT-AATACATCTG

Probes 10–14 were designed within our laboratory. The remaining probes are from the MRC Holland kit (SALSA MLPA P236-A3 ARMD Kit).

Probes spanning the duplication.