Table 7.
Hematology data of main groups in the 90-day gavage study of 100 nm zinc oxide nanoparticles*
| Negative control | Vehicle control | 31.25 mg/kg | 125 mg/kg | 500 mg/kg | |
|---|---|---|---|---|---|
| n# | 10 | 10 | 10 | 10 | 10 |
| ZnOAE100(−) | |||||
| Male | |||||
| Eosinophils (%) | 1.20±0.4 | 1.40±0.6 | 1.50±0.5 | 1.80±0.6 | 2.60±0.9b,d |
| Total erythrocyte (106 cells/μL) | 8.30±0.26 | 8.53±0.31 | 8.47±0.33 | 8.57±0.31 | 9.29±0.52b,d |
| Hemoglobin (g/dL) | 14.4±0.4 | 14.5±0.6 | 14.1±0.5 | 14.1±0.4 | 12.8±0.8b,d |
| Ht (%) | 44.4±1.4 | 44.9±1.8 | 44.1±1.5 | 44.1±1.5 | 41.7±1.8b,d |
| MCV (fL) | 53.4±1.2 | 52.6±1.3 | 52.1±2.0 | 51.5±1.5a | 45.0±3.6b,d |
| MCH (pg) | 17.3±0.4 | 17.0±0.4 | 16.7±0.7 | 16.5±0.5a | 13.9±1.4b,d |
| MCHC (g/dL) | 32.4±0.2 | 32.3±0.2 | 32.0±0.4 | 32.0±0.4 | 30.8±0.8b,d |
| PT (seconds) | 15.7±0.7 | 15.7±0.8 | 15.1±0.7 | 15.2±0.5 | 14.3±1.0b,d |
| Female | |||||
| Eosinophils (%) | 1.60±0.6 | 1.90±0.8 | 1.90±0.6 | 2.30±1.3 | 3.10±1.3a |
| Hemoglobin (g/dL) | 13.9±0.8 | 14.0±0.3 | 13.9±0.6 | 13.8±0.8 | 12.9±0.8c |
| MCV (fL) | 54.2±1.5 | 54.7±1.5 | 54.7±1.8 | 54.3±1.6 | 50.2±2.7b,d |
| MCH (pg) | 17.7±0.5 | 17.9±0.6 | 17.8±0.6 | 17.6±0.7 | 15.7±1.0b,d |
| MCHC (g/dL) | 32.6±0.5 | 1.85±0.49 | 32.5±0.6 | 32.3±0.5 | 31.4±0.8b,d |
| ZnOAE100(+) | |||||
| Male | |||||
| Hemoglobin (g/dL) | 14.3±0.7 | 14.5±0.6 | 14.4±0.4 | 14.0±0.5 | 12.5±0.9b,d |
| Ht (%) | 44.4±2.0 | 45.3±1.6 | 44.5±1.5 | 43.8±1.6 | 40.8±2.3b,d |
| MCV (fL) | 51.9±1.0 | 52.2±1.8 | 51.2±1.5 | 50.5±1.8 | 46.1±2.4b,d |
| MCH (pg) | 16.7±0.6 | 16.7±0.6 | 16.6±0.5 | 16.1±0.7 | 14.1±1.0b,d |
| MCHC (g/dL) | 32.3±0.5 | 32.1±0.5 | 32.4±0.6 | 31.9±0.3 | 30.7±0.6b,d |
| PLT (103 cells/μL) | 1,019±104 | 1,045±112 | 1,070±113 | 1,054±112 | 1,248±228a |
| PT (seconds) | 15.5±0.7 | 15.7±0.7 | 15.5±1.0 | 15.4±1.0 | 14.3±0.6b,d |
| Female | |||||
| Total leukocyte count (103 cells/μL) | 3.02±0.91 | 3.22±0.7 | 3.12±0.83 | 3.53±0.96 | 6.11±1.61b,d |
| MCV (fL) | 55.2±3.0 | 54.3±1.7 | 53.9±1.5 | 53.9±1.5 | 48.2±3.8b,d |
| MCH (pg) | 18.1±0.8 | 17.9±0.6 | 17.7±0.5 | 17.7±0.5 | 15.0±1.4b,d |
| MCHC (g/dL) | 32.8±0.4 | 32.9±0.5 | 32.8±0.3 | 32.8±0.3 | 30.9±1.4b,d |
| PLT (103 cells/μL) | 1,056±132 | 1,078±101 | 1,158±112 | 1,158±112 | 1,296±225b,d |
Notes:
*
Data are given as mean ± standard deviation;
#
number of animals. Significantly different from negative control (aP<0.05, bP<0.01) and vehicle control (cP<0.05, dP<0.01) by Scheffé’s test.
Abbreviations: Ht, hematocrit; MCH, mean cell hemoglobin; MCHC, mean cell hemoglobin concentration; MCV, mean cell volume; PLT, platelet; PT, prothrombin time; ZnO, zinc oxide; ZnOAE100(−), 100 nm negatively charged ZnO; ZnOAE100(+), 100 nm positively charged ZnO.