Table 8.
Hematology data of recovery groups in the 90-day gavage study of 100 nm zinc oxide nanoparticles*
| Negative control | Vehicle control | 500 mg/kg | |
|---|---|---|---|
| n# | 5 | 5 | 5 |
| ZnOAE100(−) | |||
| Male | |||
| Neutrophils (%) | 15.2±3.6 | 18.8±2.6 | 23.5±4.9a |
| Lymphocytes (%) | 79.6±3.7 | 75.6±3.8 | 69.5±6.8a |
| Total erythrocyte (106 cells/μL) | 8.44±0.44 | 8.20±0.23 | 9.28±0.61a,d |
| Hemoglobin (g/dL) | 14.3±0.5 | 13.5±0.3 | 13.3±0.5a |
| MCV (fL) | 52.2±1.6 | 51.6±0.5 | 47.8±2.2b,d |
| MCH (pg) | 16.9±0.7 | 16.5±0.1 | 14.4±1.1b,d |
| MCHC (g/dL) | 32.5±0.4 | 31.9±0.4 | 30.1±1.1b,d |
| APTT (seconds) | 18.8±1.1 | 18.2±1.7 | 20.6±0.7c |
| Female | |||
| Monocytes (%) | 3.4±0.6 | 2.7±0.5 | 2.0±0.3b |
| Total erythrocyte (106 cells/μL) | 7.63±0.48 | 7.45±0.20 | 8.24±0.38c |
| MCV (fL) | 55.8±2.1 | 54.3±1.1 | 50.8±2.0b,c |
| MCH (pg) | 18.2±0.6 | 17.9±0.5 | 16.3±1.0b,c |
| ZnOAE100(+) | |||
| Male | |||
| Total erythrocyte (106 cells/μL) | 8.35±0.27 | 8.28±0.19 | 9.40±0.48b,d |
| MCV (fL) | 53.1±1.1 | 52.9±1.2 | 48.8±1.6b,d |
| MCH (pg) | 17.4±0.3 | 17.1±0.4 | 14.8±0.8b,d |
| MCHC (g/dL) | 32.7±0.3 | 32.3±0.3 | 30.3±1.1b,d |
| Female | |||
| Total erythrocyte (106 cells/μL) | 7.38±0.67 | 7.52±0.30 | 8.18±0.31d |
| MCH (pg) | 18.5±0.7 | 17.8±0.9 | 16.4±0.7b,c |
| MCHC (g/dL) | 32.9±0.5 | 32.1±0.4 | 31.2±0.5b,c |
Notes:
*
Data are given as mean ± standard deviation;
#
number of animals. Significantly different from negative control (aP<0.05, bP<0.01) and vehicle control (cP<0.05, dP<0.01) by Scheffé’s test.
Abbreviations: APTT, activated partial thromboplastin time; MCH, mean cell hemoglobin; MCHC, mean cell hemoglobin concentration; MCV, mean cell volume; ZnO, zinc oxide; ZnOAE100(−), 100 nm negatively charged ZnO; ZnOAE100(+), 100 nm positively charged ZnO.