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. 2014 Jul 3;4(3):e954870. doi: 10.4161/21592780.2014.954870

Figure 1.

Figure 1.

Endosome-to-Golgi transport of Kex2 requires Ypt31/32, but not Trs85. (A) Deletion of TRS85 does not affect the punctate distribution of the Golgi protein Kex2-YFP. Kex2 was tagged with YFP at its C-terminus in wild type, trs85Δ, and ypt31Δ/ypt32ts mutant cells. The intra-cellular distribution of Kex2-YFP was determined by live-cell microscopy. Shown from top to bottom: YFP, DIC, and% cells with puncta (N, number of cells visualized for each strain). In trs85Δ mutant cells, like in wild type cells, Kex2-YFP shows punctate distribution. As previously shown,18 in ypt31Δ/32ts mutant cells, which are defective in endosome-to-Golgi transport, Kex2-YFP is diffuse. (B) Deletion of TRS85 does not affect the level of Kex2-YFP. The level of Kex2-YFP protein in cells from panel A was determined using immuno-blot analysis and anti-GFP antibodies. From top to bottom: Kex2-YFP, loading control (G6PDH), and ratio of Kex2-YFP/loading control (+/−, STD). The level of Kex2-YFP in trs85Δ mutant cells is similar to that of the wild type, whereas it is lower in ypt31/32ts mutant cells, as previously shown.18 Results shown in this figure represent 2 independent experiments.