Table 1.

Sequences of the oligonucleotide primers used in this study

Pathogen	Target gene	Primers		Final amplicon size (bp)	References
		Primer name	Oligonucleotide sequences (5’-3’)
Anaplasma & Ehrlichia	16S rRNA	EC9	TACCTTGTTACGACTT	1462	Kawahara et al., 2006 [10]
		EC12A	TGATCCTGGCTCAGAACGAACG
A. bovis	16S rRNA	AB1f	CTCGTAGCTTGCTATGAGAAC	551	Kawahara et al., 2006 [10]
		AB1r	TCTCCCGGACTCCAGTCTG
A. phagocytophilum	16S rRNA	SSAP2f	GCTGAATGTGGGGATAATTTAT	641	Kawahara et al., 2006 [10]
		SSAP2r	ATGGCTGCTTCCTTTCGGTTA
A. marginale	msp4	Amargmsp4 F	CTGAAGGGGGAGTAATGGG	344	Torina et al., 2012 [11]
		Amargmsp4 R	GGTAATAGCTGCCAGAGATTCC
A. ovis	msp4	Aovismsp4 F	TGAAGGGAGCGGGGTCATGGG	347	Torina et al., 2012 [11]
		Aovismsp4 R	GAGTAATTGCAGCCAGGGACTCT
Hemoparasite	18S rRNA	Primer A	AACCTGGTTGATCCTGCCAGT	1750	Medlin et al., 1988 [14]
		Primer B	GATCCTTCTGCAGGTTCACCTAC
Theileria	18S rRNA	989	AGTTTCTGACCTATCAG	1100	Allosop et al., 1993 [15]
		990	TTGCCTTAAACTTCCTTG
T. luwenshuni	18S rRNA	Tl310	GGTAGGGTATTGGCCTACTGA	340	Yin et al., 2008 [16]
		Tl680	TCATCCGGATAATACAAGT
Babesia	18S rRNA	Babesia F	TGTCTTGAATACTT(C/G)AGCATGGAA	950	Ramos et al., 2010 [17]
		Babesia R	CGACTTCTCCTTTAAGTGATAAC