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. Author manuscript; available in PMC: 2015 May 27.
Published in final edited form as: Nature. 2014 Nov 27;515(7528):577–581. doi: 10.1038/nature13988

Figure 1. Mutations in Lama4 and Alg8 form top predicted d42m1-T3 epitopes.

Figure 1

a, Growth of d42m1-T3 or F244 tumours in 5-mouse cohorts treated with αPD-1 (closed circles), αCTLA-4 (open circles), αPD-1+αCTLA-4 (open triangle) or control mAb (closed triangle). b, Potential H-2Kb binding epitopes predicted by in silico analysis of all missense mutations in d42m1-T3. c, Median affinity values for the top 62 predicted H-2Kb epitopes. d, Median affinity values of H-2Kb epitopes after filtering. e, Screening for specificities of CD8+ TILs from αPD-1 treated, d42m1-T3 tumour bearing mice using H-2Kb tetramers loaded with top 62 H-2Kb epitopes. f, IFN-γ and TNF-α induction in CD8+ TILs from αPD-1 treated, d42m1-T3 tumour bearing mice following culture with irradiated splenocytes pulsed with the top 62 H-2Kb peptides. Data are presented as per cent CD8+ TILs expressing IFN-γ, TNF-α or for both. Data are representative of two independent experiments.