Extended Data Figure 7. CD8⁺ T cells specific for mutant forms of Lama4 and Alg8 infiltrate d42m1-T3, but not F244, tumours.

a, Detection of tumour infiltrating mLama4- or mAlg8-specific T cells infiltrating d42m1-T3 or F244 tumours of mice treated with αPD-1. Tumours were harvested on day 12 post-transplant. Cells were gated on live CD45⁺ and CD8α⁺ tumour infiltrating lymphocytes. Detection of mLama4- or mAlg8-specific T cells was achieved by staining with peptide-MHC H-2K^b PE-labelled tetramers. Data are representative of at least five independent experiments. b, Detection of mLama4-specific tumour infiltrating T cells from tumour-bearing mice treated with αPD-1, αCTLA-4, both αPD-1 plus αCTLA-4 or control mAb. Detection of mLama4-specific T cells was achieved by staining with mLama4-MHC H-2K^b PE-labelled tetramers. Data presented are plotted as the mean mLama4 tetramer-positive as a percent of CD8α⁺ tumour infiltrating cells and are representative of at least three independent experiments.