Extended Data Figure 3. mLama4 and mAlg8 stimulate CD8⁺ T cell lines generated against d42m1-T3 following αPD-1.

a, CD8⁺ T cell lines generated from splenocytes of individual d42m1-T3 tumour bearing mice that rejected their tumours after αPD-1 therapy were incubated with irradiated d42m1-T3 tumour cells (or F244 tumour cells) treated with blocking mAb specific for H-2K^b, and/or H-2D^b and IFN-γ production quantitated. Data are presented as means ± s.e.m and are representative of two independent experiments. Samples were compared using an unpaired, two-tailed Student’s t test (***p<0.001). b, IFN-γ release by the CTL 74 T cell line following co-culture with naïve irradiated splenocytes pulsed with the top 62 H-2K^b synthetic peptides added at 1 μM final concentration.