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. 2014 Dec 19;14:374. doi: 10.1186/s12870-014-0374-4

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© Ranf et al.; licensee BioMed Central. 2014

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Calcium elevation is reduced after treatment with flg22 (A), elf18 (B) and AtPep1 (C) but not with chitin octamer (ch8) (D) in all five cce5 mutants. To enable absolute cytosolic calcium measurements, the parental HVA1 (C24) line was transformed with the pMAQ2 construct (designated as HVA1-P) and used as a reference. The indicated genotypes were crossed with HVA1-P (i.e. a C24 Arabidopsis accession expressing cytosolic apoaequorin). Seedlings from the F2 populations were screened for the cce phenotype to identify homozygous cce5 plants, the mutations verified by CAPS marker analysis, and the progenies used for calcium measurements. Error bars represent standard errors of the mean.