Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2014 Dec 19;14:374. doi: 10.1186/s12870-014-0374-4

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© Ranf et al.; licensee BioMed Central. 2014

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

PMC Copyright notice

MAMP/DAMP-induced calcium elevations in T-DNA insertion mutants of PBL1 , BIK1 , PBL2 and PBS1 . T-DNA insertion mutants of pbl1, bik1 or a pbl1bik1 double mutant were crossed with the pMAQ2 aequorin transgenic line. After verifying the mutant genotypes by PCR, changes in calcium levels induced by the indicated MAMPs were measured in the F2 or F3 generations (A). Due to silencing when crossed to the pMAQ2 reporter for the pbl2 (B) and pbs1 (C) mutants, crosses were made with an independently generated pUBQ-AEQ line with the expression of the apoaequorin reporter driven by the UBIQUITIN-10 promoter. Hence, the parental pUBQ-AEQ line in Col-0 background was used as a reference for the wild type calcium signature for pbl2 and pbs1. (Two independent pbl2 mutant alleles were included). Error bars represent standard errors.