Skip to main content
. 2014 Dec 26;88(1):1–7. doi: 10.4174/astr.2015.88.1.1

Fig. 3.

Fig. 3

Immunocytochemistry staining of indirect cocultures. (A) On day 1, indirect coculture, AFP cells (ABC method, DAB developing, ×200), Positive: light brown staining. (B) On day 5, indirect coculture, ALB cells (S-P method AEC developing, ×200), Positive: reddish brown staining. (C) On day 7, indirect coculture, cytokeratin 18 cells (ABC method, DAB developing, ×200), Positive: light brown staining. (D) L02 hepotocytes ALB cells (S-P method, AEC developing, positive control, ×200), Positive: red stain. (E) Undifferentiated human multipotent adult progenitor cells AFP immunocytochemistry (ABC method, DAB developing, no light brown staining, negative control, ×200). ABC, avidin-biotin complex; DAB, diaminobezidin; S-P, streptavidin-perosidase; AEC, 3-amino-9-ethylcarbazole.