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. 2014 Dec 17;14:192. doi: 10.1186/1471-2261-14-192

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© Zhang et al.; licensee BioMed Central. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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The mRNA expression of PCSK9 (A), LDLR (B), SREBP-2 (C) and HNF1α (D) in the liver were measured using real-time PCR. The relative ratio and standard deviation were calculated using comparative CT method (ΔΔCT value). All results were normalized against GAPDH. *P < 0.05 vs. control. PCSK9 = proprotein convertase subtilisin/kexin type 9; LDLR = low-density lipoprotein receptor; SREBP-2 = sterol regulator element–binding protein-2; HNF1α = hepatocyte nuclear factor 1α; GAPDH = glyceraldehyde 3-phosphate dehydrogenase.