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. Author manuscript; available in PMC: 2014 Dec 30.
Published in final edited form as: Methods Cell Biol. 2014;119:261–276. doi: 10.1016/B978-0-12-416742-1.00013-5

FIGURE 14.3.

FIGURE 14.3

Stem cell adhesion study using combinatorial fibronectin patterns to evaluate cell attachment and spreading. (A) A tilted polymer pen array (180 μm pen spacing) was used to generate 15 ×15 array of fibronectin features spaced by 4 μm. MSCs were cultured on these patterns for 1 week and subsequently stained for alkaline phosphatase (ALP) (green), actin (red), and the nucleus (blue). The labels on the resulting patterns shown in (B) indicate the x-position of patterns of a certain feature size across a substrate. The total area presented to the cell can be obtained by squaring the average protein feature size and multiplying by 225 for a 15 ×15 array of dot features.