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DNA-PKcs −/− FSC cells (closed circle) and the same cells transfected with functional DNA-PKcs+/+ (T53), (closed square) were cultured for more than 20 passages in vitro and irradiated. The fraction of cells surviving irradiation is shown in Panel A. Panel B; same as in panel A, except FSC and T53 cells were obtained from F2–F3 generation tumors, from which a single cell suspension was prepared, plated and irradiated 18–24 hours later. Confidence intervals are one standard deviation.
