Fig. 1.

Expression and localization of Lyn, Fyn, Src and Fgr in mouse platelets. (A) Whole cell lysates were prepared from spleen, lung or washed platelets of control and different members of Src family kinase (Yes, Hck and Fgr)-deficient mouse as described in the Methods. Expression of the different Src family kinase members (Yes, Hck, Fgr, Blk and Lck) was detected by western blot using specific antibodies. Data are representative of at least three independents experiments. (B) The protein level of Fgr, Fyn, Lyn and Src were quantitated by densitometry in mouse platelets as described in the Methods. Representative western blot is shown in the left panel. (C) Resting and collagen-related peptide (CRP)-stimulated platelets were lysed in 1% Brij 58 lysis buffer and lipid raft were isolated by sucrose gradient ultracentrifugation. The lipid rafts fractions were detected using an anti-LAT and localization of Fgr, Fyn, Lyn and Src was detected using specific antibodies. Data are representative of two independents experiments.