Fig. 3.

Impact of double deficiency of Src family kinases (SFK) in glycoprotein VI (GPVI)-induced platelet aggregation. (A) Washed platelets (2 × 10⁸ mL⁻¹) prepared from fyn^−/−lyn^−/− (top), fgr^−/−lyn^−/− (top), fyn^−/−src^−/− (middle) or lyn^−/−src^−/− (bottom) mice and their respective litter-matched control were stimulated with different doses of CRP (0.3, 1 and 3 μg mL⁻¹). Platelet aggregation was measured as a change in light transmission and ATP secretion was measured as luciferin/luciferase-mediated luminescence, using a lumi-aggregometer. Representative images are shown (n = 4 mice per condition). (B) Time to 25% of aggregation was calculated and data are shown as mean ± SEM. One-way anova test, *P < 0.05, **P < 0.01 vs. wild type (WT).