Fig. 4.

Impact of double deficiency of Src family kinases (SFK) in protein tyrosine phosphorylation in response to glycoprotein VI (GPVI) stimulation. Washed platelets (2 × 10⁸ mL⁻¹) from fyn^−/−src^−/−, lyn^−/−src^−/− or from fyn^−/−lyn^−/− mice in comparison to their respective litter-matched controls were activated for 3 min with collagen-related peptide (CRP) (3 μg mL⁻¹) in the presence of lotrafiban (10 μmol L⁻¹) and lysed. Whole platelet lysates were immunobloted with anti-phosphotyrosine antibody. The FcRγ-chain was observed on long exposure of whole cell lysates. Syk and PLCγ2 were immunoprecipitated from whole platelet lysates, and immunoprecipitates were immunoblotted with an anti-phosphotyrosine antibody and Syk or PLCγ2 antibodies (A) Representative blots of three independent experiments are shown. (B) Data represent mean ± standard error of the mean (SEM). One-way anova test, *P < 0.05, **P < 0.01 vs. respective controls.