Fig. 6.

Impact of double deficiency of Src family kinases (SFK) in thrombus formation on collagen fibers under flow conditions. Anticoagulated blood from lyn^−/−fyn^−/−, fyn^−/−src^−/− or lyn^−/−src^−/− and their respective litter-matched controls was perfused through collagen-coated capillary tubes at 1000 s⁻¹ for 4 min. Differential interference contrast (DIC) images of fixed platelets on collagen fibers after being flowed through collagen-coated capillary tubes at 1000 s⁻¹ for 4 min (scale bar: 20 μm) are representative pictures of thrombus formed from at least three independent experiments of each genotype. Area covered by platelet thrombi and the percentage of thrombi with a different surface were measured in each of at least three different experiments and mean ± standard error of the mean (SEM) is shown. Student's t-test, *P < 0.05 vs. wild-type (WT) control.