Table 1. Platelet count, volume and surface glycoprotein expression in control and deficient platelets.
Control | fyn−/−lyn−/− | fgr−/−lyn−/− | Chimeric control | fyn−/−src−/− | lyn−/−src−/− | |
---|---|---|---|---|---|---|
PC, 103 mm−3 | 366 ± 42 | 823 ± 47 | 267 ± 49** | 744 ± 52 | 813 ± 32 | 481 ± 49** |
MPV, μm3 | 5.5 ± 0.1 | 6.2 ± 0.2** | 6.3 ± 0.2** | 5.4 ± 0.1 | 5.2 ± 0.1 | 5.7 ± 0.1 |
GPIb | 50.4 ± 1.0 | 50.8 ± 4.9 | 37.9 ± 3.5* | 41.2 ± 2.5 | 44.5 ± 3.3 | 36.2 ± 1.9* |
GPIIb | 89.5 ± 12.8 | 108.9 ± 15.6 | 104 ± 23 | 103.5 ± 17.0 | 118.3 ± 12.3 | 89.6 ± 7.9 |
GPVI | 16.7 ± 1.0 | 22.2 ± 1.6 | 15.9 ± 0.5 | 19.9 ± 2.4 | 18.5 ± 2.5 | 16.7 ± 0.4 |
α2 | 3.6 ± 0.5 | 3.3 ± 0.4 | 3.7 ± 1.0 | 3.4 ± 0.3 | 3.6 ± 0.1 | 3.1 ± 0.4 |
Whole blood platelet count and mean platelet volume (MPV) for control and deficient mice platelets were analyzed with an automated blood analyzer (ABX Pentra 60). Results are expressed as means ± standard error of the mean (SEM) for at least six mice per group. Expression of glycoproteins on the platelet surface was determined by flow cytometry using specific fluorescein isothiocyanate-labeled antibodies. Results are expressed as median fluorescence intensity ± SEM for at least four mice per group. One-way anova.
P < 0.05,
P < 0.01 vs. respective control.