Figure 4. Characterization of GAL4 expression patterns.

Flies from a GAL4 strain of interest are crossed to flies carrying the reporter gene UAS-CD8-GFP. Animals are dissected at the wandering third instar larval stage or as adults. Whole nervous systems are fixed, stained, mounted, and imaged. In larvae, γ neurons bifurcate to innervate both dorsal and medial lobes. In adults, γ neurons innervate a broad medial lobe, the α′/β′ neurons bifurcate to innervate FasII-negative dorsal and medial lobes, and the α/β neurons bifurcate to innervate FasII-positive dorsal and medial lobes. Green: anti-CD8. Red: anti-FasII.