Figure 5. Expression patterns of GAL4 enhancer trap lines in the fly brain.

Each GAL4 strain was used to drive a UAS-CD8-GFP reporter gene (green) and brains were dissected at the wandering third instar larval stage (WL3) and adult. All samples were counterstained with an antibody to FasII (red) to label the developing axon scaffold and specific mushroom body lobes: γ in larva, γ (low) and α/β (high) in adult. OK107-GAL4 is expressed in the entire mushroom body throughout development. Reporter gene expression in larval γ neurons is indicated where present. In adult brain, G0451 labels γ; G0050, 6-54, and c305a label α′/β′; c708a labels pα/β (pioneer α/β); G0391 labels oα/β (outer/earlier born α/β); and c44a labels iα/β (inner/later born α/β) neurons.