Figure 6. Amino acid properties under positive (red) and negative (green) selection in vertebrate Hh coding sequences.

Two different signifcance levels are shown: (Z-score > |1.64|) to detect significant selective signatures and (Z-score > |3.09|) to detect strong selective signatures. Amino acid properties are classified as chemical (C), structural (S) and other (O), according to da Fonseca et al. [54].