Table 2. LAMP and qPCR primers used in this study.
Assay | Primers | Sequence (5′-3′) | Gene location (bp) |
smcL_LAMP | F3 | GATTTAATTGCACAAGCGGACT | 391–412 |
B3 | CGACAATAGGCCACTGGC | 608–625 | |
FIP | ACGGGTGTTTGATGAGGATACATTT-GTCGTCATTTTAAACGAAGCCTTTG | (441–465) (397–421) | |
BIP | TGGGAGAAGTAAACATGGTTGGG-TAACGACAGCCACTCCAC | (490–507) (424–446) | |
LF | TAGTCGATGTGAAGCACTTGT | 468–488 | |
LB | CGGAAGGAAATTATTCCAATTTTGC | 553–557 | |
smcL_qPCR | F | ACCCGTAATTGGGAGTAAACAT | 518–529 |
Probe | HEX-CAGCCACTCCACCATCTTCCAAAGCA-BHQ | 574–599 | |
R | TGTTGGCTTTTTTCGAGAATAGG | 608–640 |