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. 2014 Apr 15;306(11):E1264–E1273. doi: 10.1152/ajpendo.00438.2013

Fig. 4.

Fig. 4.

Accumulation of apoB in the hepatic endoplasmic reticulum (ER) induces ER stress. A: total mRNA was extracted from McA cells either containing a control vector or stably expressing a truncated form of apoB WT or mutant, and mRNA transcripts of the sarcoendoplasmic reticulum ATPase (SERCA) gene were measured. B and C: relative mRNA expression of SERCA detected by qRT-PCR in livers of PPARα+/+ and PPARα−/− mice (B) and rats fed Chow or Fruc diets for 3 wk (C). D: relative mRNA expression of C/EBP homologous protein (CHOP) detected by qRT-PCR in livers of PPARα+/+ and PPARα−/− mice. E: immunoblot analysis of the ER stress markers phospho-eukaryotic initiation factor-2α (eIF2α), Grp94, or Grp78 in livers of PPARα+/+ and PPARα−/− mice and quantification of their signal intensity. F: relative mRNA expression of CHOP detected by qRT-PCR in livers of rats fed Chow or Fruc diets for 3 wk. G: immunoblot analysis of phospho-eIF2α and its total protein in livers of rats fed Chow or Fruc diet for 3 wk. For animal studies, results are shown as means ± SE (n = 5–6/group). For in vitro studies, data represent 3 experiments. *P < 0.05; **P < 0.01 vs. controls.