Figure 5. Induction of PGE2 formation and expression of COX-2 and CD36 in HeLa and A549.

Cells were incubated without (DMSO-treated control) or with 33 µM C18∶2n−6 or C18∶3n−6 for 20 h. Subsequently, cells were stimulated with the TLR2 agonist PAM2CSK4 or TNF-α for further 8 h. A: PGE2 in supernatants was quantified by EIA technique and is expressed as -fold increase over the unstimulated DMSO-treated control. Means ± SEM. B: Percentage of COX-2 positive cells were determined by intracellular staining and flow cytometric analysis. C: Representative histograms of HeLa and A549 incubated without (DMSO-ctrl.) or with 33 µM C18∶2n−6 or C18∶3n−6 for 4 h and stained for CD36. Markers were set in reference to the isotype controls (dotted lines).