Figure 3. miR-29a binds to 3′UTR of TAB1.
HeLa cells were co-transfected with 300 ng 3′UTR TAB plasmid (A) and 30 ng renilla construct in the presence of 30 nM miR-29a, 30 nM cont-miR or vehicle only (untr) (B). Similarly, HeLa cells were co-transfected with 3′UTR TAB construct and renilla in the presence of 500 nM Target Protect binding to seed A - TP A, or seed B - TP B or both - TP A+B (C).