Table 2. Molecular characteristics of KPC-producing clinical isolates in Ontario, 2008/2011 (N = 30).
| Isolate/yeara | KPC variant | Tn4401 isoform | S1-PFGE bandsb | Replicon types foundc | KPC-plasmid (estimated size, Kb)c | Other ß-lactamase detected |
| Kpn GN25/08 | KPC-3 | b | 3 | IncFrep, IncI2 | IncFrep (120), IncI2(80) | TEM-1, SHV-11 |
| Kpn GN26/08 | KPC-2 | a | 4 | IncA/C, IncFIIA | IncFIIA (100) | TEM-1, SHV-11 |
| Kpn GN27/08 | KPC-2 | a | 4 | IncA/C, IncFIIA | IncFIIA (100) | TEM-1, SHV-11 |
| Kpn GN66/08 | KPC-3 | b | 2 | IncI2 | IncI2 (70) | TEM-1, SHV-11 |
| Kpn GN178/09 | KPC-3 | a | 2 | IncFIIA | IncFIIA (170) | TEM-1, SHV-11 |
| Kpn GN203/09 | KPC-2 | a | 2 | IncFIIA | IncFIIA (110) | TEM-1, SHV-11 |
| Kpn GN346/09 | KPC-2 | a | 3 | IncA/C, IncFIIA | IncFIIA (190) | TEM-1, SHV-11 |
| Kpn GN446/10 | KPC-2 | a | 3 | IncFIIA, IncI2 | IncFIIA (80) | TEM-1, SHV-11 |
| Kpn GN447/10 | KPC-2 | a | 3 | IncFIIA | IncFIIA (80) | TEM-1, SHV-11 |
| Kpn GN458/10 | KPC-2 | a | 3 | IncFIIA | IncFIIA (80) | TEM-1, SHV-11 |
| Kpn GN460/10 | KPC-2 | a | 3 | IncFIIA | IncFIIA (100) | TEM-1, SHV-11 |
| Kpn GN515/10 | KPC-2 | a | 3 | IncFIIA | IncFIIA (100) | TEM-1, SHV-11 |
| Kpn GN517/10 | KPC-2 | b | 2 | IncN, IncFIIA | IncN (50) | OXA-1, SHV-11 |
| Kpn GN524/10 | KPC-2 | b | 2 | IncN, IncFIIA | IncN (50) | OXA-1, SHV-11 |
| Kpn GN542/10 | KPC-2 | a | 3 | IncA/C, IncFIIA | IncFIIA (190) | TEM-1, SHV-11 |
| Kpn GN618/10 | KPC-2 | a | 3 | IncFIIA | IncFIIA (100) | TEM-1, SHV-11 |
| Ror GN581/10 | KPC-2 | b | 5 | IncFrep | IncFrep (70) | Neg |
| Kpn GN632/11 | KPC-3 | b | 2 | IncI2 | IncI2 (80), untypeable (120) | TEM-1, SHV-11 |
| Kpn GN649/11 | KPC-2 | a | 3 | IncFIIA | IncFIIA (100) | TEM-1, SHV-11 |
| Kpn GN653/11 | KPC-2 | a | 4 | IncA/C, IncFIIA | IncFIIA (100) | TEM-1, SHV-11 |
| Kpn GN685/11 | KPC-2 | a | 3 | IncFIIA | IncFIIA (100) | TEM-1, SHV-11 |
| Kpn GN686/11 | KPC-2 | a | 2 | IncFIIA | Untypeable (80) | TEM-1, SHV-11 |
| Kpn GN689/11 | KPC-2 | a | 3 | IncFIIA | IncFIIA (100) | TEM-1, SHV-11 |
| Kpn GN702/11 | KPC-2 | a | 3 | IncFIIA | IncFIIA (100) | TEM-1, SHV-11 |
| Kpn GN710/11 | KPC-2 | a | 4 | IncA/C, IncFIIA | IncFIIA (100) | TEM-1, SHV-11 |
| Kpn GN744/11 | KPC-3 | b | 3 | IncN | IncN (70) | TEM-1, SHV-11 |
| Kpn GN803/11 | KPC-2 | a | 2 | IncA/C, IncFIIA | IncFIIA (190) | TEM-1, SHV-11 |
| Ecl GN743/11 | KPC-3 | b | 3 | IncHI2 | Untypeable (120) | TEM-1 |
| Ecl GN799/11 | KPC-3 | b | 1 | Untypeable | Untypeable (50) | TEM-1, CTX-M-15, OXA-1 |
| Cfr GN801/11 | KPC-2 | b | 3 | IncA/C | IncA/C (180), untypeable (200) | TEM-1 |
a
Kpn, Klebsiella pneumoniae; Ror, Raoultella ornithinolytica; Ecl, Enterobacter cloacae; Cfr, Citrobacter freundii.
b
Number of bands detected in the S1-PFGE gels, representing one plasmid each.
c
Clinical isolates were negative for all the 18 plasmid replicons tested using Carattoli et al approach [20] and their plasmids were defined as untypeable.