Figure 7. Translocation of glucose transporters GLUT4 in the plasma membrane upon activation of P2Y₆R.

(A) Immunoblotting is used to determine the changes in the membrane levels of the GLUT4 transporter. This is a representative blot from three individual experiments. Key to lanes: 1–3, C2C12 myotubes; 4–6, 3T3-L1 adipocytes. 1 and 4, controls; 2 and 5, MRS2957, 100 nM; 3 and 6, 200 nM insulin. (B) GLUT4/β-actin ratio based on the quantitation of the bands.