Table 2. Average DNA quantitation values1 for experiments with buccal cells.
Incubation Conditions | ||||||||
Incubation Time | Buccal Cells Shaken 56°C | Buccal Cells Stationary 56°C | Buccal Cells Shaken 65°C | Buccal Cells Stationary 65°C | Buccal Cells Shaken w/Re-suspension 56°C | Buccal Cells Stationary w/Re-suspension 56°C | Buccal Cells Shaken w/Re-suspension 65°C | Buccal Cells Stationary w/Re-suspension 65°C |
1 Hour | 0.54±0.06 | 0.26±0.02 | 0.20±0.03 | 0.17±0.04 | 1.32±0.51 | 0.37±0.08 | 0.50±0.09 | 0.32±0.02 |
3 Hours | 0.31±0.07 | 0.30±0.02 | 0.60±0.01 | 0.13±0.02 | 0.71±0.25 | 0.87±0.15 | 0.85±0.06 | 0.75±0.10 |
18 Hours | 0.44±0.11 | 0.38±0.07 | 0.65±0.01 | 0.43±0.06 | - | - | - | - |
24 Hours | 0.29±0.04 | 0.11±0.02 | 0.15±0.04 | 0.17±0.09 | - | - | - | - |
All values are expressed in ng/µL. Extraction for one hour at 56°C with shaking without re-suspension N = 3. All other extractions without re-suspension N = 2. Extractions with re-suspension N = 3.