Table 3. Average DNA quantitation values1 for experiments with blood cells.
Incubation Conditions | ||||||||
Incubation Time | Blood Cells Shaken 56°C | Blood Cells Stationary 56°C | Blood Cells Shaken 65°C | Blood Cells Stationary 65°C | Blood Cells Shaken w/Re-suspension 56°C | Blood Cells Stationary w/Re-suspension 56°C | Blood Cells Shaken w/Re-suspension 65°C | Blood Cells Stationary w/Re-suspension 65°C |
1 Hour | 0.64±0.33 | 0.56±0.05 | 0.49±0.10 | 0.89±0.10 | 1.87±0.42 | 1.56±0.13 | 1.20±0.30 | 1.30±0.18 |
3 Hours | 1.45±0.33 | 0.56±0.17 | 1.47±0.02 | 1.09±0.03 | 2.10±0.27 | 2.6±0.35 | 2.11±0.19 | 2.18±0.05 |
18 Hours | 1.18±0.15 | 0.65±0.14 | 0.78±0.18 | 1.06±0.33 | - | - | - | - |
24 Hours | 0.32±0.10 | 0.24±0.01 | 0.08±0.03 | 0.07±0.02 | - | - | - | - |
All values are expressed in ng/µL. Extraction for one hour at 56°C with shaking without re-suspension N = 3. All other extractions without re-suspension N = 2. Extractions with re-suspension N = 3.