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Representative photomicrographs showing p-ERK expression (green) in the PVN of animals injected once a day with 6 doses of saline (6 Saline), single dose of LPS (100 μg/kg ip; 5 Saline + 1 LPS), or repeated doses of LPS (6 LPS), followed by icv injections of vehicle or PTP1B inhibitor (3 nmol/rat, 5 μl) 90 min after LPS injections, and a subsequent icv injection of leptin (10 μg/rat) 30 min after. Scale bar, 100 μm.
