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. 2014 Dec 30;9(12):e116423. doi: 10.1371/journal.pone.0116423

Figure 11. Expression and phosphorylation of Src, Akt, and MAPKs signaling pathways in ChEC.

Figure 11

Expression and phosphorylation of Src and Akt were analyzed by Western blotting (A). A similar level of phosphorylated and total Src and Akt was observed in TSP1+/+ and TSP1−/− choroidal EC. Expression and phosphorylation of ERKs, JNK and p38 MAP kinases were analyzed by Western blotting (B). Please note minimal impact of TSP1-deficincy on phosphorylation and expression of ERKs in ChEC. A significant increase in phosphorylation of STAT3 was observed in TSP1−/− ChEC, while total level of STAT3 was not affected. These experiments were repeated with two different isolations of cells with similar results.