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. Author manuscript; available in PMC: 2016 Jan 1.
Published in final edited form as: Arthritis Rheumatol. 2015 Jan;67(1):96–106. doi: 10.1002/art.38883

Figure 2.

Figure 2

Blocking KCa1.1 channels inhibits the proliferation and pro-MMP-2 production by PIA-FLS without inducing cell death. A, Dose-dependent inhibition of PIA-FLS proliferation by paxilline (left) and iberiotoxin (right). Mean ± SEM; n = 4 independent experiment ran in triplicate. B, Effects of blockers of KCa1.1 (20 μM paxilline; 50 mM TEA), other KCa (100 nM apamin; 1 μM TRAM-34), and Kv (100 nM ShK-186; 1 μM PAP-1) channels on the proliferation of PIA-FLS. n = 3 independent experiments, each ran in triplicate and shown as mean ± SEM. C, Dose-dependent toxicity of staurosporine (striped bars) and paxilline (black) after a 48 hr incubation with PIA-FLS. Mean ± SEM; n = 3 independent experiment ran in duplicate. D, Dose-dependent inhibition of pro-MMP-2 production by paxilline (left) and TEA (right), measured by gelatin gel zymography in PIA-FLS supernatants. Mean ± SEM; n = 3 independent experiment ran in duplicate. *p<0.05; **p<0.01; ***p<0.001.