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. Author manuscript; available in PMC: 2016 Jan 15.

Published in final edited form as: Virology. 2014 Dec 8;475:204–218. doi: 10.1016/j.virol.2014.11.020

Fig. 5 — Analysis by Western blot and immunogold labeling of vaccinia cores after treatment with high salt. A) The equivalent of 0.06 A_260nm units (~0.7 μg) of virus that was incubated in NP-40 + DTT to prepare cores as described in Materials and Methods. Cores were then incubated in buffer S (Cntl) or buffer SN (NaCl) as described in Materials and Methods and labeled on the top of the figure. The presence of specific protein in the pellet (P) or supernatant (S) fraction was determined by Western blot. B) Vaccinia virus were adsorbed on grids and incubated with NP-40+DTT to prepare cores as described in Materials and Methods. After this treatment, the grids were incubated in buffer S (A–D) or buffer SN (E–H) for 20 minutes, washed in ddH₂O and proteins associated to the virions were identified by immunogold labeling using the antibodies A10 (●) A4 (•) (A, E), A10 (●) F17 (•) (B, F), A10 (●) H1 (•) (C, G), A10 (●) A3 (•) (D, H) as described in Materials and Methods.

Fig. 5 — Analysis by Western blot and immunogold labeling of vaccinia cores after treatment with high salt. A) The equivalent of 0.06 A_260nm units (~0.7 μg) of virus that was incubated in NP-40 + DTT to prepare cores as described in Materials and Methods. Cores were then incubated in buffer S (Cntl) or buffer SN (NaCl) as described in Materials and Methods and labeled on the top of the figure. The presence of specific protein in the pellet (P) or supernatant (S) fraction was determined by Western blot. B) Vaccinia virus were adsorbed on grids and incubated with NP-40+DTT to prepare cores as described in Materials and Methods. After this treatment, the grids were incubated in buffer S (A–D) or buffer SN (E–H) for 20 minutes, washed in ddH₂O and proteins associated to the virions were identified by immunogold labeling using the antibodies A10 (●) A4 (•) (A, E), A10 (●) F17 (•) (B, F), A10 (●) H1 (•) (C, G), A10 (●) A3 (•) (D, H) as described in Materials and Methods.