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Temperature sensitive mutant in the core wall protein A3 make fragile particle under non-permissive conditions. Purified Cts8 virions grown at the non-permissive temperature were adsorbed on a Formvar/carbon coated grid and incubated in the presence of Tris (A), or NP-40 + DTT (B–F). After 20 min, the grids were washed, and two were incubated with 40 μg/ml DNase (C, F), for 15 min. The grids were stained with Nano-W (A–C) or UA (D–F) as described in Materials and Methods.
