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. Author manuscript; available in PMC: 2016 Jan 1.
Published in final edited form as: Hepatology. 2014 Nov 25;61(1):171–183. doi: 10.1002/hep.27380

Fig. 5. Loss of SRSF3 increases insulin/insulin-like growth factor signaling.

Fig. 5

(A) Igf2 and Igf2bp3 mRNA expression by Q-PCR. T indicates tumor and NT indicates non-tumor part of HKO liver.. (B) IGF2 immunostaining of WT and HKO liver at 24 mo (brown). (C) Analysis of exon 11 skipping in Insr transcripts from livers at 24 mo and from Srsf3:floxed hepatocytes after Adv-cre infection. Representative gels are shown with the % exon11 skipping underneath. (D) Activation of insulin and IGF2 signaling in primary hepatocytes. Cells were stimulated with 30 (+) or 100 nM (++) insulin or 50 (+) or 100 ng/ml (++) IGF2, then extracts were immunoblotted for phospho and total IR, Akt and Erk1/2. (E) IGF2-stimualted proliferation of WT and HKO hepatocytes by MTT assay. (F) Igf2 and Igf1 mRNA expression by Q-PCR following Adv-cre infection of Srsf3:floxed hepatocytes or siRNA knockdown in HepG2 cells.