Skip to main content
. Author manuscript; available in PMC: 2015 Sep 7.
Published in final edited form as: Cell Transplant. 2014 Mar 7;24(8):1599–1614. doi: 10.3727/096368914X678571

Figure 1.

Figure 1

During anti-CD3/-CD28 Ig stimulation of splenocytes extracted from C57BL/6 mice, the percentage of B-cells positive for TIM4 (B220+TIM4+) (n=3, *p<0.05 and **p<0.01 vs. D0; A) and of DCs positive for TIM4 (CD11c+TIM4+) decreases over time (n=3, *p<0.05 vs. D0; B). A reduction in B-cells positive for CD40 and CD80 and an increase in B-cell positive for CD86 and CD69 was also observed at day 2 after stimulation (n=3, *p<0.05, **p<0.01, and ***p<0.001 vs. D0; C). No difference in term of apoptosis was observed in TIM4+ and TIM4− B-cells at day 2 after stimulation (D). A reduction in the percentage of TIM4+ B-cells was observed after anti-CD40-Ig or LPS stimulation of isolated cells (n=3, *p<0.05 vs. D0; E). An increase in DCs positive for CD86 and CD69 was also observed at day 2 after stimulation (n=3, **p<0.01 vs. D0; F). No difference in term of apoptosis was observed in TIM4+ and TIM4− DCs at day 2 after stimulation (G). A reduction in the percentage of TIM4+ DCs was observed after anti-CD40-Ig or LPS stimulation of bone marrow derived DCs (n=3, *p<0.05 and **p<0.01 vs. D0; H). The percentage of B-cells positive for TIM4 decreases over time in splenocytes extracted from NOD mice and stimulated with the islet peptides BDC2.5 (n=3, *p<0.05 and **p<0.01 vs. D0; I). The percentage of DCs positive for TIM4 remained unchanged in splenocytes extracted from NOD mice stimulated with BDC2.5 (J).