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. Author manuscript; available in PMC: 2016 Jan 1.
Published in final edited form as: Biochimie. 2014 Nov 18;108:108–119. doi: 10.1016/j.biochi.2014.11.007

Figure 2.

Figure 2

Human IVD UV/Visible spectra with bound CoA persulfide: (A) Spectrum of 44 µM IVD and a near equivalent amount of IBD and SCAD with bound CoA-persulfide. (B) IVD with bound CoA persulfide in the presence of increasing amounts (13, 51, 75, and 103 µM) of isovaleryl-CoA to CoA persulfide-bound IVD. Scans were performed under anaerobic conditions in 50 mM Tris, pH 8.0, at ambient temperature.