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Transgenic RBCs expressing the KEL2B antigen were transfused every 4 weeks (for a total of 3 transfusions) into Townes mice homozygous for Hgb SS, heterozygous for Hgb S (AS), or homozygous for Hgb A (AA). A Anti-KEL glycoprotein Igs were measured by flow cytometric crossmatch 28 days after the first transfusion, and B measured again 28 days after the 3rd and final transfusion.
